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Preprint:
Bulk RNA sequencing dataset of hippocampi from RORγt transgenic mice and wild-type littermates
Tetsuya Sasakia,c,d,*, Sae Sanakaa,b, Kenyu Nakamuraa,b, Suguru Iwataa,d, Momo Morikawaa,d, Yosuke Takeia,c,d
Abstract
This dataset comprises bulk RNA-sequencing data from bilateral hippocampi of six untreated, 10-week-old male mice: three heterozygous RORγt-transgenic mice and three wild-type littermates on a C57BL/6 background. Total RNA was extracted using TRIzol, and RNA integrity numbers ranged from 8.1 to 8.6. Ribosomal RNA-depleted, directional libraries were prepared from 500 ng of RNA per sample and sequenced on an Illumina NextSeq 500 using 2 × 36-bp paired-end reads. The raw dataset contains 48 compressed FASTQ files representing four partitions with two read directions for each of six biological samples. Individual read totals range from 26.21 to 30.47 million per sample. Gene-level quantification against the mm10 reference using CLC Genomics Workbench version 10.1.1 is provided as complete count and reads per kilobase of transcript per million mapped reads matrices, each containing 49,585 feature entries. This article describes sample identifiers, file organization, experimental procedures, mapping summaries, and verification of file integrity and processed-data consistency. The dataset supports independent processing and comparative analyses of hippocampal gene expression. The dataset is deposited in NCBI Gene Expression Omnibus under accession GSE347403.
Keywords: Neuroimmune interactions; Rorc; Mus musculus; Paired-end sequencing; Gene expression matrix; Data reuse
